Real Madrid have become the first club in any sport to generate more than 500 million euros in revenue in a single year, according to the business advisory firm Deloitte.
The company's Football Money League, published for the 16th time on Thursday, sees Spanish champions Madrid retain their hold on first place ahead of perennial rivals Barcelona.
In an unchanged top six, Manchester United remain third, Bayern Munich fourth, European champions Chelsea fifth and Arsenal sixth, but English champions Manchester City soar five places to seventh.
AC Milan fall one place to eighth, with Liverpool remaining ninth and Italian champions Juventus rising three places to complete the top 10.
Madrid's revenue rose by seven percent to 512.6 million euros in the 2011-2012 season, equivalent to £414.7 million or $644.7 million at June 2012 exchange rates.
It enabled the nine-time European champions to hold onto top spot in the ranking for an eighth successive year, matching United's record from 1996-1997 to 2003-2004.
"It is an impressive achievement for Real Madrid to have surpassed 500 million euros in revenue in a single year," said Dan Jones from Deloitte's Sports Business Group.
"Real have led the way in the phenomenal rate of revenue growth achieved by the game's top clubs, with the double-digit (10-per cent) increase by the top 20 clubs representing continued strong performance in these tough economic times.
"The combined revenues of the top 20 clubs have quadrupled since we began our analysis in 1996-1997."
Madrid triumphed in the Spanish Liga in 2011-2012, ending three years of dominance by arch-rivals Barcelona, but lost out in the Champions League after being beaten by Bayern in the semifinals.
Overall, the combined revenue of the world's 20 highest-earning football clubs grew by 10 percent on the previous year to reach 4.8 billion euros.
City's climb of five places was matched by German champions Borussia Dortmund, who rose to 11th, and Italian side Napoli (15th).
"Manchester City's Premier League title-winning season, combined with participation in the UEFA Champions League, helped drive 51-percent revenue growth to 285.6 million euros, the largest absolute and relative growth of any Money League club," said Deloitte's Austin Houlihan.
"The club's progress to the top of the English and European game means that they are set to remain a top 10 Money League club for the foreseeable future."
The only new entry in the list was English side Newcastle United, who moved into the top 20 at the expense of Spanish club Valencia after a surprise fifth-place finish in the Premier League last season.
The English top flight remains the most well-represented league in the ranking, with seven of the 20 clubs hailing from the Premier League.